Mouse anti-V5 tag mAb sepharose Purification kit
Affinity purified anti-V5 antibodies, developed in mouse, were conjugated to
NHS-sepharose. It is an efficient technique for isolating recombinant proteins
or mammalian expression proteins. The V5 epitope system relies on the
peptide (N-Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-C),
which is able to react with N- and C- terminal V5-tagged fusion proteins and
may be used for the immunoprecipitation or immune affinity purification.
The purified antibody is immobilized at 3-5 mg antibody per mL of 50% slurry
and this kit allows a rapid and efficient affinity purification of active V5 fusion
proteins. The affinity resin allows an efficient binding of V5 fusion proteins
without the need for preliminary steps and calibrations. The affinity bound V5
fusion proteins can be efficiently eluted from the resin by acid condition. The
eluted proteins can be used for characteristic analysis.
NHS-sepharose. It is an efficient technique for isolating recombinant proteins
or mammalian expression proteins. The V5 epitope system relies on the
peptide (N-Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-C),
which is able to react with N- and C- terminal V5-tagged fusion proteins and
may be used for the immunoprecipitation or immune affinity purification.
The purified antibody is immobilized at 3-5 mg antibody per mL of 50% slurry
and this kit allows a rapid and efficient affinity purification of active V5 fusion
proteins. The affinity resin allows an efficient binding of V5 fusion proteins
without the need for preliminary steps and calibrations. The affinity bound V5
fusion proteins can be efficiently eluted from the resin by acid condition. The
eluted proteins can be used for characteristic analysis.
Component:
Note: Sepharose is 1:1 suspension in Phosphate Buffered Saline, pH 7.4, containing 0.05% sodium azide as a preservative.
Product capacity:
The binding and elution capacity of 1 mL Mouse anti-V5 tag mAb sepharose are commonly more than 1 mg of V5 fusion proteins. Trying different elution buffers for optimal results is recommended.
Materials Required but Not Provided:
For sustainable use and long term storage, store at 2 °C to 8 °C. DO NOT FREEZE.
Shipping Conditions:
Blue ice
| Reagent | Quantity for 10 rxns (C07003-K02) |
Quantity for 5 rxns (C07003-K01) |
composition |
| Mouse anti-V5 tag mAb sepharose | 2 mL X 1 vial | 1 mL X 1 vial | 50% slurry of Mouse anti-V5 tag mAb sepharose in 1X Phosphate Buffered Saline |
| Wash Buffer (10X concentration) |
5.0 mL X 1 vial | 2.5 mL X 1 vial | 100 mM Tris/Cl pH 7.5; 1.5 M NaCl; 5 mM EDTA |
| Elution Buffer | 10 mL X 1 vial | 5 mL X 1 vial | 0.1 M Glycine pH 2.7 |
| Neutralization Buffer | 2 mL X 1 vial | 1 mL X 1 vial | 2 M Tris pH 8.0 |
| spin column | 10 pcs | 5 pcs | |
| collection tube | 10 tubes | 5 tubes |
Product capacity:
The binding and elution capacity of 1 mL Mouse anti-V5 tag mAb sepharose are commonly more than 1 mg of V5 fusion proteins. Trying different elution buffers for optimal results is recommended.
Materials Required but Not Provided:
- Micro-centrifuge capable of 15,000 x g
- 1.5 mL Centrifuge tubes
- End-over-end rotator
- CoIP Lysis Buffer (mild reaction): 10 mM Tris/Cl pH 7.5; 150 mM NaCl; 0.5 mM EDTA; 0.5% NP-40
- RIPA (vigorous reaction): 100 mM Tris/Cl pH 7.5; 300 mM NaCl; 0.2% Sodium Deoxycholate (or 0.1% SDS); 2% NP-40
For sustainable use and long term storage, store at 2 °C to 8 °C. DO NOT FREEZE.
Shipping Conditions:
Blue ice